Alkaline lysis method of plasmid isolation was given by which s... | Filo
Alkaline lysis method of plasmid isolation was given by which s... | …
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Brianna Bibel on Instagram: "Minipreps (alkaline lysis)! A practical guide to what goes on in each of the steps Aka ALKALINE LYSIS, a “miniprep” is a technique in which we separate and purify the plasmid DNA we put into bacteria (the DNA we want) from all the stuff that was already in the bacteria (the DNA we don’t want, proteins, sugars, etc.). You can’t just break the cells open (lyse them) & pull out *all* the DNA because the bacteria has its own DNA which you aren’t interested in. But some c
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