Digital PCR (dPCR) is a rediscovered method that researchers use for the absolute quantification of nucleic acid samples. Compared to quantitative real-time PCR, dPCR is more sensitive, precise, and ...
With standard PCR systems, users are required to set a predefined quantity of cycles according to assay and sample-specific factors. Single-cell sequencing tests demand knowledge of the number of ...
Real-time PCR plays a foundational role in gene expression studies, disease analysis, and molecular diagnostics. However, success depends on precise reaction setup, optimized assays, and accurate ...