Young scientists often get frustrated with laborious experimental procedures, such as cloning, PCR, or sequencing. However, the Internet has made planning and performing these procedures faster and ...

While the traditional cloning workflow has been used in laboratories for decades, new techniques have emerged that allow for rapid assembly of multiple genetic fragments. The Electra Vector System, ...

One of the most indispensable techniques in all areas of the life sciences is the use of expression vectors. Cloning a gene between two restriction sites, and the expression of a protein, are almost ...

CHO cells carrying the 21ΔpqHAC vector were maintained in Ham’s F-12 nutrient mixture (Invitrogen, Carlsbad, CA, USA) with 10% fetal bovine serum (FBS: JRH Biosciences, Lenexa, KS, USA) and 8 μg/ml ...